#CARLO TRAVERSO GENOVA FREE#

The chosen experimental model allowed to show that the rod OS is a primary producer of oxidative stress linked to the pathogenesis of degenerative retinal diseases.

#CARLO TRAVERSO GENOVA FREE#

Here we show that Manool, a diterpene extracted from Salvia tingitana has the potential to lower the free radical production by light-exposed rod outer segments in vitro, by specifically targeting the rod OS F 1F o-ATP synthase belonging to the extramitochondrial OxPhos expressed on the disk membrane. In conclusion, data are consistent with the antioxidant properties of Salvia spp., suggesting its ability to lower oxidative stress production, a primary risk factor for degenerative retinal diseases. Moreover, the overall slowdown of OxPhos metabolism reduced the ROI production elicited in the OS by light exposure, in vitro. Dive into the research topics where Carlo Traverso is active. Results confirm that the OxPhos machinery is ectopically expressed in the OS and that F 1F o-ATP synthase is a target of Manool, which inhibited the OS ATP synthesis, binding the F 1 moiety with high affinity, as analysed by molecular docking. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA). As diterpenes display an antioxidant effect, we have evaluated the effect Manool, extracted from Salvia tingitana, on the extramitochondrial OxPhos and the ROI production in the retinal rod OS. In fact, OS conduct aerobic metabolism, producing ATP through oxygen consumption, although it is devoid of mitochondria. However, a Reactive Oxygen Intermediates (ROI) production in the retinal photoreceptor layer seems to depend also on the expression of an extramitochondrial oxidative phosphorylation (OxPhos) machinery in the rod outer segments (OS). Mitochondria are considered the principal source of oxidative stress. Uncontrolled oxidative stress production, especially in the outer retina is one of the causes of retinal degenerations.

Carlo traverso genova